Filter, Fluorescence

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JDSU builds fluorescence filter sets for applications in fluorescence microscopy and other biomedical applications. In one of the more common applications, a filter set is composed of an excitation filter, an emission filter, and a dichroic beamsplitter. A filter set may be designed to work with a single fluorophore or with multiple fluorophores simultaneously.

The superior precision and repeatability of the new Ucp-1 filter deposition platform enables the realization of filters with exceptional blocking levels, high transmission levels, and very steep transition zones. The practical result in fluorescence based applications is bright, high-contrast images.

JDSU’s Ucp-1 platform is a magnetron-sputtering-based deposition system. The high throughput of the system is enabled by a high material deposition rate  combined with the incorporation of a load lock which reduces chamber down time. Coatings deposited with the Ucp-1 platform are fully dense and robust, and have the same durability as coatings deposited using other energetic film deposition processes such as ion beam sputtering and ion-assisted deposition.Ucp-1 coatings pass the environmental durability tests cited in ISO-9211-3 Category C, as well as extended cyclic humidity testing as described in MIL-STD-810F (5 cycles) without measurable change in their spectral characteristics.

Key Features

  • High peak transmission
  • Deep blocking over wide spectral ranges
  • Steep transition slopes
  • Precise edge placement
  • Low autofluorescence
  • Hard dielectric coatings that are robust and reliable
  • Single substrate, epoxy free optical path
  • High brightness
  • Large signal to noise ratio/high contrast
  • Customer-specified sizes and mounting options available

Applications

  • Fluorescence microscopy
  • Confocal microscopy
  • Flow cytometry
  • Genotyping
  • Microplate readers
  • Imaging systems
  • Spectroscopy

Contacts

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